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. 2016 Feb 17;7:105. doi: 10.3389/fpls.2016.00105

Table 2.

Pigment analysis during the first 6 h of HL.

0 h 0.5 h 2 h 6 h
Chl a/b 3.210 ± 0.014 3.231 ± 0.005 3.240 ± 0.020 3.241 ± 0.006
Chl/Car 4.193 ± 0.007 4.036 ± 0.019 3.981 ± 0.017 3.740 ± 0.013
Neo/100 Chls 3.269 ± 0.139 3.440 ± 0.095 3.416 ± 0.080 3.613 ± 0.193
Vio/100 Chls 3.157 ± 0.181 1.930 ± 0.067 1.804 ± 0.081 1.822 ± 0.101
Ant/100 Chls 0.416 ± 0.015 0.634 ± 0.021 0.725 ± 0.017 1.001 ± 0.042
Lut/100 Chls 14.989 ± 0.654 15.921 ± 0.257 15.772 ± 0.338 16.218 ± 0.643
Zea/100 Chls 0.274 ± 0.015 1.129 ± 0.053 1.599 ± 0.154 2.296 ± 0.129
β-Car/100 Chls 1.739 ± 0.939 1.720 ± 0.372 1.797 ± 0.619 1.781 ± 1.125
Cars/100 Chls 23.846 ± 0.041 24.776 ± 0.118 25.116 ± 0.109 26.734 ± 0.096
(Z + 0.5 × A)/(Z + A + V) 0.125 ± 0.003 0.391 ± 0.004 0.474 ± 0.010 0.546 ± 0.002

Pigments were extracted from thylakoids from plants grown under GL200 and transferred for 0.5, 2 and 6 h. The chlorophyll a/b (Chl a/b) ratio and chlorophyll/carotenoid (Chl/Car) ratio was determined the same way from isolated thylakoid membranes in three different repetitions (n = 3). The same extracts were used for the quantification by HPLC of the carotenoids: neoxanthin (Neo), violaxanthin (Vio), anteraxanthin (Ant), zeaxanthin (Zea), lutein (Lut) and β-carotene (β-car). All carotenoids were calculated per 100 Chls.