Table 2.
0 h | 0.5 h | 2 h | 6 h | |
---|---|---|---|---|
Chl a/b | 3.210 ± 0.014 | 3.231 ± 0.005 | 3.240 ± 0.020 | 3.241 ± 0.006 |
Chl/Car | 4.193 ± 0.007 | 4.036 ± 0.019 | 3.981 ± 0.017 | 3.740 ± 0.013 |
Neo/100 Chls | 3.269 ± 0.139 | 3.440 ± 0.095 | 3.416 ± 0.080 | 3.613 ± 0.193 |
Vio/100 Chls | 3.157 ± 0.181 | 1.930 ± 0.067 | 1.804 ± 0.081 | 1.822 ± 0.101 |
Ant/100 Chls | 0.416 ± 0.015 | 0.634 ± 0.021 | 0.725 ± 0.017 | 1.001 ± 0.042 |
Lut/100 Chls | 14.989 ± 0.654 | 15.921 ± 0.257 | 15.772 ± 0.338 | 16.218 ± 0.643 |
Zea/100 Chls | 0.274 ± 0.015 | 1.129 ± 0.053 | 1.599 ± 0.154 | 2.296 ± 0.129 |
β-Car/100 Chls | 1.739 ± 0.939 | 1.720 ± 0.372 | 1.797 ± 0.619 | 1.781 ± 1.125 |
Cars/100 Chls | 23.846 ± 0.041 | 24.776 ± 0.118 | 25.116 ± 0.109 | 26.734 ± 0.096 |
(Z + 0.5 × A)/(Z + A + V) | 0.125 ± 0.003 | 0.391 ± 0.004 | 0.474 ± 0.010 | 0.546 ± 0.002 |
Pigments were extracted from thylakoids from plants grown under GL200 and transferred for 0.5, 2 and 6 h. The chlorophyll a/b (Chl a/b) ratio and chlorophyll/carotenoid (Chl/Car) ratio was determined the same way from isolated thylakoid membranes in three different repetitions (n = 3). The same extracts were used for the quantification by HPLC of the carotenoids: neoxanthin (Neo), violaxanthin (Vio), anteraxanthin (Ant), zeaxanthin (Zea), lutein (Lut) and β-carotene (β-car). All carotenoids were calculated per 100 Chls.