Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 17;7:162. doi: 10.3389/fmicb.2016.00162

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Lozano, Trenado, Fiallo-Olivé, Chirinos, Geraud-Pouey, Briddon and Navas-Castillo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Southern blot analysis to detect the presence of DNA satellites in a sweepovirus-infected sweet potato sample (B3). Total DNA (∼1 μg) was separated on 0.8% agarose gel electrophoresis in TAE, transferred to a positively charged nylon membrane and hybridized with digoxigenin-labeled probes synthesized by PCR from clone SBG53 (satellite, left panel) and the sweet potato leaf curl virus (SPLCV) isolate present in that sample (virus, right panel). Total DNA was digested (right part of each panel) with a restriction enzyme which recognizes a unique site in the satellite (PstI) and SPLCV (BamHI). The positions of open circular (oc), supercoiled (sc), and single-stranded (ss) DNA forms are indicated with black arrowheads. The positions of linear (lin) DNA forms are indicated with white arrowheads. Mobility of the size marker (1 kb DNA ladder) is given in the right margin.