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. 2016 Feb 17;6:21721. doi: 10.1038/srep21721

Figure 7. Luciferase reporter gene assay.

Figure 7

(a) Luciferase reporter gene assay using COS-7 cells transfected with p53-Luc Cis-reporter Plasmid. COS-7 cells were transiently transfected with Pathdirect p53-Luc Cis-reporter Plasmid. For negative control vector, pCIS-CK supplied by manufactures was used. FLAG-Pemt-HA (Pemt), myc-CHC (CHC) and p53-HA (p53) plasmids were co-expressed in COS-7 cells. Overexression of Pemt and CHC additively reduced p53-luciferase activities. Data are means ± SE. n = 5 in all groups. **P < 0.01. (b,c) Luciferase reporter gene assay using H-4-II-E-C-3 cells treated with shRNA-CTRL or shRNA-Pemt. H-4-II-E-C-3 cells were transiently transfected with Pathdirect p53-Luc Cis-reporter Plasmid and treated with 25 mM high glucose (HG), 100 nM insulin, 20 ng/ml IL-6, or 250 μM Palmitate for 24 hr. In H-4-II-E-C-3 cells, the treatments with shRNA-Pemt increase p53-luciferase activities compared to shRNA-CTRL. Palmitate and insulin stimulate p53-luciferase activities in shRNA-Pemt-treated H-4-II-E-C-3 cells. Data are means ± SE. n = 5 in all groups. **P < 0.01.