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. 2016 Feb 17;6:21721. doi: 10.1038/srep21721

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Copyright © 2016, Macmillan Publishers Limited

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(a) Quantification of DNA methylation using Imprint Methylation DNA Quantification Kit. In Pemt−/− under HFHS diet, methylated DNA increased compared to Pemt+/+ mice. (b) mRNA expression profiling by DNA chip analysis. The numbers of gene with RNA expression ratio (Pemt−/− to Pemt+/+) over 2 or under 0.5 are shown at 1, 2, 3 and 4 weeks after the feeding with HFHS diet. (c) Western blot analyses of F-box protein 31 (Fbxo31) and hepatocyte nuclear factor 4 alpha (HNF4α) in liver samples from Pemt+/+, Pemt+/− and Pemt−/− mice.