Table 1. List of primers used in this study.
| Primer | Sequence | Remark |
|---|---|---|
| Cloning | ||
| codY’ fw | TTTTAAGCTTGAAGAGCAATTGGCAGAAGAA | Hind III |
| codY’ rv | TTTTGAATTCGCAATCACAGAAGCAGTCAAT | EcoR I |
| ‘a’: codYtest1 | CATCAGTCGTATAGGTCAGAT | / |
| ‘b’: codYtest2 | AATGACGTTTATCCATCCAAC | / |
| ‘c’: pSET4Stest2 | AATGACGTTTATCCATCCAAC | / |
| ‘d’: pSET4Stest1 | TCGCTATTACGCCAGCTG | / |
| X-f: | TCTCTACACAAAGTGGGCTACC | |
| X-r: | GACTGGCTCGCAACCTTCTT | |
| Y-f: | CCGATAGAAGAAGCAGCCTCT | |
| Y-r: | ACGGCACACTTAAATCCTTTGA | |
| CodY Overexpression | ||
| his-codY1 fw | TTTTCCATGGGCCATCATCATCATCATCACAGCATGACAACATTATTAGAGAAGACAC | Nco I |
| his-codY1 rv | TCTTGGATCCTTAGTAGTCACGTTTCTTAATTTCAT | BamH I |
| qRT- PCR | ||
| ilvH-F | GAGTGAGGTGCTACATCGACAACAT | SSU05_1887 |
| ilvH-R | AACAAATCATCAAACAGCTAAACCG | |
| bap2-F | CTTTACGACGACGGCTGGCTTGATT | SSU05_0780 |
| bap2-R | GTGATGATATTGTTCAAACCGAGGT | |
| glo-F | CCAATCATCGAAACGGAACCAATC | SSU05_0725 |
| glo-R | TATGATAGGTCAAGCCAAGCAACTG | |
| guaA-F | CCTTTTTCGGCGGTGGACAGGACTA | SSU05_1472 |
| guaA-R | TAATATTGACCGAGCAACTGGAAGC | |
| acc-F | TGGCTTCAATGGGCACAGTGGT | SSU05_1797 |
| acc-R | GTAAAGAGGACGACTGGCAAAC | |
| cps2J-F | ACGCAGAGCAAGATGGTAGAATAAAA | SSU05_0573 |
| cps2J-R | CAAGTAACCCTCCCGACAAATCACTAT | SSU05_0573 |
| 16S RNA-F | ATGGACCTGCGTTGTATTAGC | |
| 16S RNA-R | CATTGCCGAAGATTCCCTAC | |
*Underlined regions in the primers represent the cleavage sites for the restriction enzymes mentioned in the remarks column; qRT- PCR, Quantitative Real Time PCR.