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. 2016 Feb 17;6:21465. doi: 10.1038/srep21465

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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C57Bl/6NCrlBR mice were infected with 5 × 10⁶ CFU/lung of planktonic bacteria for the acute infection or 1 to 2 × 10⁶ CFU/lung of strains embedded in agar beads for the chronic infection and analyzed during a time course post-infection (12 hours and 1 day of acute infection and 2, 14 and 28 days of chronic lung infection). Cytokines and chemokines, including MIP-2 (A), KC (B), MIP-1α (C), IL-6 (D), MCP-1 (E) and TNF-α (F), were measured by Bioplex in lung homogenates. The data are pooled from at least two independent experiments (n = 3–6). Values represent the mean ± SEM. Statistical significance is indicated: *P < 0.05, **P < 0.01.