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. 2016 Feb 15;7:10654. doi: 10.1038/ncomms10654

Figure 1. Co-expression network of GAME9 in potato and tomato.

Figure 1

(a) Using RNA-Seq transcriptome data from potato and tomato, we found that GAME9 was co-expressed with most of the SGA biosynthetic genes. A total of 1,260 and 168 genes were co-expressed with GAME9 in tomato and potato, respectively. Out of them, thirty seven were shared homologs co-expressed in both potato and tomato (see Supplementary Data 1 and Supplementary Table 1). C5-SD: Δ(7)-STEROL-C5(6)-DESATURASE, SMO1: METHYLSTEROL MONOOXYGENASE 2-2-LIKE, OXR: 3-β HYDROXYSTEROID DEHYDROGENASE, CAS: CYCLOARTENOL SYNTHASE. (b) Normalized expression profile (from RNA-sequencing) of GAME9 in different tomato tissue types and developmental stages. Br, breaker; IG, immature green; MG, mature green; Or, orange; R, ripe. FPKM: Fragments Per Kilobase of transcript per Million mapped reads. (c) In situ mRNA hybridization showing GAME9 expression in wild-type tomato shoot meristems. Both longitudinal and transverse sections are shown. Sense GAME9 probe is shown as a control. Arrows indicate higher expression in the vascular system and the outer layer of the leaf blade. Scale bars: 200 μm.