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. 2016 Feb 15;7:10654. doi: 10.1038/ncomms10654

Figure 3. Changes in GAME9 expression in potato and tomato results in altered levels of the predominant SGAs.

Figure 3

(a,b) GAME9 gene expression (qRT-PCR) in GAME9-Ox (overexpression) and GAME9-RNAi (silencing) lines in potato (a) and tomato (b) leaf tissue. WT: wild-type. Potato GAME9-Ox independent lines (#7, #20, #21 and #22). Tomato GAME9-Ox independent lines (#6328 and #6359) and GAME9-RNAi (#6319 and #6355). (c–f) Levels of α-solanine and α-chaconine in leaves (c) and peel (e) of potato tubers of GAME9-Ox lines and levels of α-tomatine and dehydrotomatine in leaves (d) and green fruit (f) of GAME9-RNAi and GAME9-Ox tomato lines, determined by LC-MS. Values represent means±s.e. (n=3). Student's t-test was used to assess whether the transgenic lines significantly differ from wild-type plants: *P value<0.05; **P value<0.01; ***P value<0.001.