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. 2016 Feb 15;7:10654. doi: 10.1038/ncomms10654

Figure 7. Expression of genes involved in the synthesis of SGAs and sterol precursors in tomato leaves derived from GAME9-Ox and GAME9-RNAi lines.

Figure 7

A schematic view of the sterol and SGA biosynthetic pathways. Dashed arrows represent multiple biosynthetic reactions whereas solid arrows represent a single step. Graphs next to each gene name show expression levels in wild-type (WT), two GAME9-Ox (lines 1:#6328 and 2:#6359) and two GAME9-RNAi lines (line 3:#6319 and 4:#6355) determined by qRT-PCR. For genes where qRT-PCR data is not presented, it is due to technical reasons and differential expression awaits confirmation. According to RNA-Seq data, expression of SQS, TTS1, TTS2 and GAME2 was similar in GAME9-Ox and GAME9-RNAi tomato lines. For other genes, expression is represented based on RNA-Seq data, with arrows next to each gene depicting an increase (red) or decrease (blue) in GAME9-Ox and GAME9-RNAi lines, respectively. Values represent means±s.e. (n=3). Student's t-test was used to assess whether the transgenic lines significantly differ from wild-type plants: *P value<0.05; **P value<0.01; ***P value<0.001.