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. 2016 Feb 17;6:21714. doi: 10.1038/srep21714

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(a) The quality of AgNPs was characterized by ultraviolet detection at different time points. The peak position of the wavelength of AgNPs was stable at 396 nm during the initial 8 days. (b) Contact angle of chitosan-Ag0/heparin multilayer films versus the layer number. Heparin and chitosan almost exhibited a strict alternate layer-upon-layer arrangement. (c) UV-visible spectra of chitosan-Ag0/heparin multilayer films versus the bilayer number. All multilayer film coats at different bilayers presented a maximum absorption at 433 nm wavelength. The maximum absorption presented a linear increase with the increased number of bilayers during the assembly process.