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. 2016 Feb 17;6:21714. doi: 10.1038/srep21714

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Copyright © 2016, Macmillan Publishers Limited

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(a) The surface topography of the AgNP multilayer film coats from the dip-assembly and the spin-assembly methods, respectively, was observed with atomic force microscope (AFM). (b) The surface topography of AgNP multilayer film coats from the spin-assembly methods was observed with scanning electron microscopy (SEM). (c) The macroscopic morphology of the AgNP biliary stent is shown. (d) The influence of the AgNP biliary stent on coagulation function was evaluated based on the detection of re-calcification clotting time. The blank polyester served as the control. AgNPs-heparin: heparin as the outermost layer of the polyester; the polyester with AgNPs-chitosan: chitosan as the outermost layer of the polyester. ***p < 0.001. (e) A Ag⁺ release experiment was conducted through measurements of Ag⁺ concentration at different time points using the inductively coupled plasma mass spectrometry (ICP-MS).