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. 2016 Feb 17;6:21275. doi: 10.1038/srep21275

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Iact of early protein-restriction on hippocampal BDNF (a) and Zif268 (b) gene expression. The hippocampus was dissected from adult rats born to dams fed a control or a low-protein diet during gestation and lactation that were either undisturbed in their home cages or evaluated for Short Term Memory using the NOR task. Animals were sacrificed 2 h after the end of the learning session and the levels of the transcripts determined by real-time PCR. Values illustrate the relative abundance of each transcript in relation to those of 18S amplified within the same sample and under the same experimental conditions. *p < 0.05 compared with control naïve animals (Student’s t-test). ^§p < 0.05; ^§§§p < 0.001 (two-way ANOVA followed by Bonferroni’s multiple comparison tests). The number of animals within each group ranged from 4 to 6.