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. 2016 Feb 17;6:21315. doi: 10.1038/srep21315

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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3-dimensional (3D) reconstructions of vehicle control (a–c) and latrunculin-A (Lat-A)-treated (d–f) trabecular meshwork (TM). 3D reconstructions were cropped and rotated to show en face and oblique tissue views. Cortical filamentous actin (red: F-actin) networks were prominent amongst nuclei and autofluorescent structures (green) in vehicle controls (a–c) but not in Lat-A-treated tissue (d–f). Punctate actin collections were prominent in Lat-A-treated tissue. b,e): High magnification 3D reconstruction of TM (30 × 20 × 9 μm crop of 246 × 246 × 100 μm 3D volume) showing cortical F-actin network (closed arrows) and punctate perinuclear actin collections (open arrows) associated with autofluorescent fibers and nuclei (asterisks). (c,f): Isosurface maps of F-actin closely correspond to phalloidin-labeled F-actin phalloidin in vehicle control (b vs. c) and Lat-A-treated tissue (e vs f), capturing treatment-induced F-actin network and punctate changes. Asterisks: nuclei; closed arrows: cortical F-actin network; green fibers: autofluorescent structure; green ovals: nuclei; open arrows: punctate F-actin collection; red fluorescence: F-actin.