Fig. 2.

EMMPRIN overexpression induces phenotypic changes in normal human lung fibroblasts. Growth arrested NHLF transiently transfected with either EMMPRIN/GFP or GFP were treated with TGF- β1 5 and 10 ng/ml for 48 h. a Cell proliferation was assessed by thymidine incorporation. b Cell migration using Modified Boyden chamber. Each bar represents means ± SEM of 3 independent experiments each run in duplicates. ** p < 0.001 comparing GFP to EMMPRIN/GFP transfected cells, # p < 0.01 Comparing TGF- β1 treated cells to control. c A representative Western blot for α – smooth actin (α-SMA) of cell lysates from EMMPRIN/GFP and GFP control cells in the presence and absence of TGF-β1. β-Tubulin was used as the loading control. Image is representative of 3 independent experiments each run in duplicate