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. 2016 Feb 17;17:17. doi: 10.1186/s12931-016-0334-7

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© Hasaneen et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — EMMPRIN overexpression induces resistance of normal human lung fibroblasts to apoptosis: Growth arrested NHLF overexpressing either EMMPRIN/GFP or GFP were treated with TGF- β1 from 0 to 10 ng/ml for 24 h in the presence and absence of 0.5 mM of Staurosporine (STS) added for 3 h prior to the end of the experiments. Apoptosis was measured by FACS analysis (a-b) using annexin V/PI staining and cell death detection ELISA assay (c). Relative apoptosis is expressed as a percentage of the assay-positive control that was run on the ELISA plate for each experiment. All samples were run in triplicate for each ELISA and FACS analysis. ** p < 0.001 comparing GFP to EMMPRIN/GFP transfected cells (n = 3 independent experiments)