(A) Exploded view of smart cup for minimally-instrumented, point-of-care molecular diagnostics. The smart cup consists of a thermos cup body, a 3D-printed cup lid, a chip holder, and a smartphone adapter. Inset is a photograph of Mg-Fe alloy pouch ($ 0.15 per pouch) used as a heating source. (B) A photograph of a fully assembled, smart cup equipped with a Samsung Galaxy S3 smartphone. Inset is a photograph of an integrated microfluidic chip containing three independent, multifunctional, isothermal amplification reactors for nucleic acid extraction and amplification.