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. Author manuscript; available in PMC: 2016 Feb 17.
Published in final edited form as: Cell Rep. 2015 Nov 19;13(9):1965–1976. doi: 10.1016/j.celrep.2015.10.061

Figure 6. Altered balance between DR and MR 5-HTergic neuronal activity in PNFLX mice.

Figure 6

(A–C) Fos and 5-HT immunostaining on hM3Dq+ and hM4Di+ mice and controls in DR (A) and MR (B) demonstrates an increase of in %Fos+/5-HT+ neurons in hM3Dq+ mice when compared to control animals. Moreover, PNFLX control animals display an increase in %Fos+/5-HT+ neurons in the MR (B), but not the DR (A) when compared to PNSAL control animals. n = 3–9 per genotype, post-natal treatment and region.

(D–G) The anatomical location of in vivo electrophysiological recordings of putative 5-HTergic raphe neurons was validated at the end of each recording session through responsiveness to 5-HT1A receptor mediated inhibition of neuronal firing, achieved by 8-OH-DPAT administration (ip, 1 mg/kg) (D, F). Insets show examples of single extracellularly recorded spikes from PNSAL (plain line) and PNFLX (dashed lines) animals. The firing frequency of putative 5-HTergic neurons of PNFLX mice were reduced in the DR (E) but increased in the MR (G) when compared to PNSAL animals. n = 8–9 animals.

* p < 0.05; ** p < 0.01; *** p < 0.005. Scale bars are 20µm and 100µm. DR: dorsal raphé, MR: median raphé.