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. 2016 Feb 17;16:18. doi: 10.1186/s12896-016-0249-x

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© Klose et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Illustration of the prokaryotic and eukaryotic expression vectors. The synthetic tetanus toxoid fragment C (TTC) DNA sequence was cloned into the prokaryotic expression vector pBM and the eukaryotic expression vector pMS using the restriction sites SfiI and NotI. a Prokaryotic expression vector pBM-TTC-ETA’. pelB = signal sequence for protein secretion into the periplasm; 10xHis-tag = polyhistidin-sequence for detection and purification of recombinant proteins; A = antigen fragment; ETA’ = deletion mutant of Pseudomonas aeruginosa exotoxin A; f1 ori = origin of replication for production of single-stranded DNA by M13-helper phage; kanR = kanamycin resistance gene for he selection of transformed cells; ori(3331) = origin of replication; lacI = Lac repressor; T7 prom + Lac op. = IPTG-inducible promotor + Lac-Operator. b Eukaryotic expression vector pMS-L-SNAP-TTC. pCMV = constitutive active promotor of the cytomegalovirus; Ig-κ-Leader = murine signal sequence for protein secretion into the cell culture supernatant; Myc/His-tag = c-myc-epitope for detection/polyhistidin-tag for detection and purification; eGFP = enhanced green fluorescent protein; BGH = Bovine growth hormone (BGH) polyadenylation signal, ZeoR = Zeocin® resistance gene for the selection of transfected cells, pSV40 = early SV40-promotor, SV40 replication origin (ORI); polyA = polyadenylation signal, ColE1 origin = bacterial origin of replication; AmpR = ampicillin resistance gene for the selection of transformed Escherichia coli