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. 2016 Feb 17;16:18. doi: 10.1186/s12896-016-0249-x

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© Klose et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Binding analysis of recombinant SNAP-TTC-BG647 on polyclonally activated CD19⁺ B cells. CD19⁺ B cells were isolated from leukocyte filters by density gradient centrifugation and magnetic cell separation with anti-CD19 beads. Isolated B cells were activated by adding 2.5 μg/ml CpG and 50 ng/ml IL-21 to the cell culture medium. On day 4 of cultivation, the cells were analyzed by flow cytometry. Surface a and intracellular b staining with the recombinant SNAP-TTC-BG 647 (1:25) was performed