Fig. 8.

Cytotoxic activity of TTC-ETA’ on TTC-specific polyclonally activated memory B cells. Frequencies of IgG (a, c), of tetanus toxoid (TT)- (b) and of TTC-specific IgG (d) antibody-secreting cells (ASC) incubated with recombinant TTC (10 nM) or TTC-ETA’ (10 nM) protein on day 2 of polyclonal activation. The data are the results of 5 independent experiments using TT-coated plates (b) and 7 experiments using TTC-coated plates (d) and the corresponding total IgG ELISPOT assay (a, c). Statistical analysis was performed using the Wilcoxon matched-pairs signed rank test