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. 2016 Feb 17;16:46. doi: 10.1186/s12870-016-0736-1

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© Liu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Validation of nucleotide variations (NVs) identified by RNA sequence comparison. Gene g662 was used to illustrate the verification of NVs. The upper panel shows the cDNA sequence of g662 and the seven NVs (mark in red) identified by RNA sequence comparison of bruchid-resistant (R) and -susceptible (S) mungbean. The NVs in parentheses show the nucleotides in R mungbean (the former letter) changed to that in S mungbean (the latter letter). The lower panel shows the validation of NVs by genomic sequencing between R mungbean lines RIL59 andTC1966 and S line NM92. The color of the letter is synchronized with that of the chromatogram for easy reading. The box indicates the site of NVs. The order of NV sites starts from down-left then down-right panels