. 2016 Feb 17;17:43. doi: 10.1186/s12863-016-0350-0

Table 1.

Primer pairs used to amplify the ε-LCY and β-LCY genes. The final two columns give the size of amplicons and the used high-throughput technology (CelI/agarose gel or DHPLC method). The partial denaturation DHPLC temperatures are reported in brackets

Gene	Genome	Exon region	Primer	Sequence (5’-3’)	Amplicon size (bp)	Method
β-LCY-6A	A	1	F4f	AGCCCTACAACCCGGGA	861	CelI/agarose gel
			PC63r	CCCATGAAGATCTTGAGA
			F4f	AGCCCTACAACCCGGGA	464	DHPLC (65.8 °C)
			OP6r	GTGCGCGCCACCATGTACC
β-LCY-6B	B	1	PC71f	ATCCCGGCCACCGTCGTCCTGGA	990	CelI/agarose gel
			PC62r	CCATGAAGATCTTGAGATGC
			PC68f	GTCTTCATCGACGACCACA	761	DHPLC (65.1 °C)
			OP6	GTGCGCGCCACCATGTACC
ε-Lcy-3A	A	4–9	PC35f	TGCTGAGAAGGTAGACATTCTATTG	1,193	CelI/agarose gel
			PC40r	CAAGCATTGATGGACTGGAC
		4–5	PC35f^a	TGCTGAGAAGGTAGACATTCTATTG	650	DHPLC (57.8 °C)
			PC130r^a	CATTGCAGAAGCACACTGC
		6–7–8	PC42f^a	GGTTGATGTCTCGGTTGGAT	443	DHPLC (57.9 °C)
			PC40r^a	CAAGCATTGATGGACTGGAC
		8–9	PC43f	TGGACAATATTTGCCTGGAA	385	DHPLC (57.5 °C)
			PC37r	CTTGCGTACTCGCGAAAAA
			PC35f	TGCTGAGAAGGTAGACATTCTATTG	1,527	Used for nested PCR
			PC37r	CTTGCGTACTCGCGAAAAA
ε-Lcy-3B	B	4–9	PC42f	GGTTGATGTCTCGGTTGGAT	1,530	CelI/agarose gel
			PC46r	GCATCCTTGCGTATTGTATTCTT
		4	PC44f^a	TTGCTGAGAAGGTACATTCGAT	336	DHPLC (58.4 °C)
			PC140r^a	GGCACTTTGTGCAGGGTTGG
		5–6–7	PC41f	GAGGACCACGTGTTTGTGTG	584	DHPLC (58.1 °C)
			PC143r	ACACCTGTGCAAGATAAACC
		7–8–9	PC147f	TCCTTACCTAACACAGACCAGA	636	DHPLC (58.2 °C)
			PC48r	AAAGATACGCATCCTTGCGTATT

^aPrimer combinations required nested PCR