Table 5.
Mutation frequencies obtained through DHPLC analysis on 1,140 screened plants of the mutagenized durum wheat cv. Marco Aurelio
| Gene | Primer | Exon region | Amplicon size (bp) | Mutation n° | Mutation frequency (1/kb) | ||
|---|---|---|---|---|---|---|---|
| β-LCY-6A | F4f-OP6r | 1 | 464 | 17 | 1/31 | ||
| β-LCY-6B | PC68f-OP6r | 1 | 761 | 8 | 1/108 | ||
| ε-LCY-3A | PC35f-PC130r | 4–5 | 650 | 12 | 1/62 | ||
| PC42f-PC40r | 6–7–8 | 443 | 2 | 1/252 | |||
| PC43f-PC37r | 8–9 | 384 | 3 | 1/146 | |||
| mean | 1/102 | ||||||
| ε-LCY-3B | PC44f-PC140r | 4 | 336 | 4 | 1/95 | ||
| PC41f-PC143r | 5–6–7 | 584 | 7 | 1/95 | |||
| PC147f-PC48r | 7–8–9 | 636 | 10 | 1/72 | |||
| mean | 1/84 | ||||||
| Total/mean | 4,258 | 46 | 1/77 | ||||