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Mutation frequencies obtained through CelI/agarose method on 1,140 screened plants of the mutagenized durum wheat cv. Marco Aurelio
| Gene | Primer | Exon region | Amplicon size (bp) | Mutation n° | Mutation frequency (1/kb)a |
|---|---|---|---|---|---|
| β-LCY-6A | F4f-PC63r | 1 | 871 | 5 | 1/176 |
| β-LCY-6B | PC71f-PC62r | 1 | 990 | 6 | 1/167 |
| ε-LCY-3A | PC35f-PC40r | 4–8 | 1,193 | 9 | 1/138 |
| ε-LCY-3B | PC44f-PC46r | 4–8 | 1,530 | 11 | 1/148 |
| Total/mean | 4,584 | 31 | 1/168 |
aThe fragment size of each gene was reduced to 100 bp because of PCR artefacts
