Fig. 5.

Avidity maturation of subdomain-specific antibodies during natural HIV-1 infection. a For each distinct subdomain, four representative yeast clones were used (see Fig. 3a, c) to measure the mean fluorescence intensity (MFI) of all sequential plasma samples from P08 and P11, and from which the association (Ka) and disassociation (Kd) constants were calculated using nonlinear least squares fit. b Dynamic changes in Ka of subdomain-specific antibodies during the first 2–2.4 years of HIV-1 infection. Ka is the reciprocal of Kd