Fig. 6.

ERβ–TDG interaction affects gene regulation. a TDG increases ERβ transcriptional activity in reporter assays. Tdg^−/− MEFs were transfected with plasmids encoding for the reporter gene 3× ERE-luc, TK-renilla, ERβ, and TDG in different concentrations (50, 150, and 300 ng transfected plasmid), and treated with 10 nM E2. After 16 h, firefly luciferase activity was measured and normalised against renilla luciferase activity (means + SD; n ≥ 3). TDG co-expression increased luciferase activity significantly (** p < 0.01, *** p < 0.005). Expression of ERβ and TDG was confirmed by western blot analysis. b TDG is recruited to ERβ-regulated genes in MEFs. TDG recruitment to indicated genes in wt, βerko, and βerkohERβ MEFs was analysed by ChIP-qPCR. Asterisk indicates significant (p < 0.05) enrichment compared to binding at an unrelated, heterochromatic region on chromosome 2 [13] (means + SD; n ≥ 3)