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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Oct 31;1862(1):135–144. doi: 10.1016/j.bbadis.2015.10.028

Fig. 4.

Fig. 4

OPN is a downstream target of leptin and is PI3K/Akt regulated in primary HSC. Freshly isolated HSC were treated with leptin (or vehicle), with or without LY294002. Additional HSC were also pre-treated with Ad5dnAkt (or Ad5GFP) prior to treatment with leptin (100 ng/ml) or Ad5mryAkt alone. RNA was isolated and changes in gene expression evaluated by qRT-PCR. (A) OPN mRNA. (B) αSMA mRNA. (C) Collagen 1α1 mRNA. Results are expressed as fold changes relative to vehicle-treated HSC, and graphed as mean ± SEM. *p < 0.05 vs. vehicle.