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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Oct 31;1862(1):135–144. doi: 10.1016/j.bbadis.2015.10.028

Fig. 5.

Fig. 5

OPN neutralization abrogates fibrogenesis in precision-cut liver slices (PCLS). Precision-cut, viable mouse liver slices (PCLS) (a liver organ culture system) were stimulated with leptin (or vehicle), in the presence of sham- or OPN-specific aptamers for 48 h. At the end of treatment, liver slices were harvested, viability assessed by ATP content, and RNA analyzed by qRTPCR. (A) ATP content (viability) in nmol/mg protein; hashed line denotes the accepted viability threshold. (B) OPN mRNA. (C) αSMA mRNA. (D) Collagen 1α1 mRNA. Results are expressed as fold changes relative to vehicle-treated PCLS, and graphed as mean ± SEM. *p < 0.05 vs. vehicle.