Figure 5.

Distribution of reflection multiplicity and crystal orientation in an IMISXcryo data set recorded using 104 β₂AR crystals. (a) Blue, observed multiplicity. Red, binomial distribution of multiplicity predicted for an effective crystal rotation range of 2.5°. (b) Projection of the unit-cell a axis along the X-ray beam direction (red dot). The horizontal axis (dotted line) is the rotation-spindle direction and the vertical axis (solid line) is perpendicular to both the X-ray beam and rotation-spindle directions. Each blue diamond represents one crystal. For any given crystal in the set, the angle between its unit-cell axis and the X-ray beam direction is indicated by the position of the corresponding diamond on a circle. These range from 90° on the limiting circle to 0 or 180° at the centre of the circle. (c) Projection of the unit-cell b axis along the X-ray beam direction. (d) Projection of the unit-cell c axis along the X-ray beam direction. The skewed distribution in (a) is consistent with the preferential crystal orientation with the a axis of the unit cell close to the X-ray beam direction (b).