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. 2016 Feb 17;6:21092. doi: 10.1038/srep21092

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Pairwise variation calculated by geNorm to determine the minimum number of reference genes for accurate normalization in all the samples (Combined), or in the samples of the experimental condition of interest. Every bar represents change in normalization accuracy when stepwise adding more endogenous reference genes according to the ranking in Fig. 3. CON: conventional larvae; GF: germ-free larvae and GFPr: germ-free larvae supplemented with a probiotic candidate.