Table 1. 3′:5′ratio calculation, RQI values and RNA concentrations of LCM samples of sea bass larval intestinal tissue.

Groups	ΔCq 3′	ΔCq 5′	3′:5′ Ratio*	RQI	Conc. (pg)
GF 10 dph	7.7	8.6	2.0	[8.4]	178
GF 10 dph	8.5	8.5	1.0	[4.9]	108
GF 10 dph	7.2	7.9	1.7	[8.3]	159
GF 10 dph	8.6	8.8	1.1	[7.1]	159
GF 10 dph	6.7	5.9	0.6	7.1	378
GF 10 dph	6.9	6.7	0.9	7.7	334
GF 10 dph	8.2	9.2	2.2	[5.9]	100
GF 10 dph	8.3	9.4	2.3	7.1	303
GF 10 dph	5.8	6.6	1.8	7.3	410
GF 10 dph	6.3	7.5	2.4	[8.9]	197
GFPr 10 dph	9.1	9.3	1.3	[5.4]	69
GFPr 10 dph	7.9	8.6	1.7	[3.3]	102
GFPr 10 dph	6.7	7.2	1.4	[10]	65
GFPr 10 dph	6.7	7.4	1.7	[8.2]	57
GFPr 10 dph	8.2	9.1	2.0	[7.5]	135
GFPr 10 dph	7.9	8.4	1.5	[5]	112
GFPr 10 dph	7.2	7.8	1.6	[9.4]	171
GFPr 10 dph	7.4	7.4	1.1	[7.8]	62
GFPr 10 dph	6.3	6.4	1.1	[8.1]	207
GFPr 10 dph	8.1	7.9	0.9	7.2	284
CON 16 dph	8.0	8.4	1.4	[5.5]	139
CON 16 dph	7.4	7.1	0.8	[6.5]	164
CON 16 dph	8.0	7.8	0.9	[6.8]	128
CON 16 dph	9.2	9.4	1.2	[4.3]	121
CON 16 dph	7.0	7.0	1.0	[9.1]	170
CON 16 dph	8.6	8.4	0.9	[8.8]	100
CON 16 dph	8.5	8.4	0.9	[5.7]	114
CON 16 dph	7.9	7.8	0.9	[5.9]	125
CON 16 dph	8.9	8.6	0.8	[7.8]	95
CON 16 dph	9.1	8.8	0.8	[5.3]	105
GF 16 dph	6.8	6.0	0.6	[5.2]	167
GF 16 dph	8.0	7.4	0.7	[6.5]	139
GF 16 dph	8.0	7.0	0.5	[5.8]	105
GF 16 dph	8.8	8.4	0.8	[5.3]	144
GF 16 dph	8.1	7.5	0.7	[7.5]	105
GF 16 dph	7.1	6.9	0.9	[6.2]	168
GF 16 dph	9.1	8.4	0.6	[5.9]	112
GF 16 dph	8.8	8.3	0.7	[4.9]	106
GF 16 dph	8.5	7.6	0.5	8.9	531
GF 16 dph	5.3	5.4	1.1	8.4	421

Unreliable RQI values are depicted in brackets.

*3′:5′ Ratio = E3′^(−ΔCq 3′) / E5′^(−ΔCq 5′).

ΔCq 3′ = mean Cq 3′ samplen - mean Cq 3′ standard curve.

ΔCq 5′ = mean Cq 5′ samplen - mean Cq 5′ standard curve.

CON: conventional larvae; GF: germ-free larvae and GFPr: germ-free larvae supplemented with a probiotic candidate.