Figure 1. Analysis of ahpC gene expression.

(A) Construction of the ahpC promoter gfp bioreporter. (B) Expression from the ahpC promoter by cells grown in LB medium with and without H₂O₂ (100 μM). (C) GFP fluorescence intensity of planktonic cells and biofilms grown in LB medium at 24 and 48 h. (D) CLSM images of the ahpC::gfp reporter strain in biofilms at 24 and 48 h. All samples were analyzed in triplicate. Statistical analyses were conducted using Student’s t-test. A letter on the bar graph indicates the level of significance.