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. 2016 Feb 17;6:20999. doi: 10.1038/srep20999

Figure 1. Diagrams illustrate the targeting strategy and the creation of mice carrying SENP2Fx or SENP2 mutant allele.

Figure 1

(A) In the targeted allele, a loxP site and a pgk-neo cassette flanked by two loxP sites were inserted into intron 3 and intron 4, respectively. Mice carrying the SENP2 targeted allele were created, and crossed with the EIIa-Cre transgenic mice to generate progeny carrying the SENP2Fx or SENP2 mutant allele. (B–D) PCR analysis detected the presence of 5′ (PCR: P1–P2) and 3′ (PCR: P3–P4) loxP sites for genotyping the wild type (+/+) and heterozygous (Fx/+) mice, and examined the deletion of exon 4 (PCR: P1–P4). (E) RT-PCR analysis detected the transcripts generated from the wild type (+/+), heterozygous (+/−) and homozygous (−/−) embryos of SENP2lacZ (left panel) and SENP2 mutant (right panel). (F) Immunoblot analysis examines protein expression in SENP2+/+ and SENP2−/− embryos. Actin level is used as a loading control.