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. 2016 Feb 17;6:21088. doi: 10.1038/srep21088

Figure 6. Dimerization of SOD1 in cancer cells exposed to lipophilic NBD compounds, and SOD1 exposed to NBD compounds in vitro.

Figure 6

(a) Western blot analysis of proteins in MDA MB468 cells pre-incubated with NAC (5 mM), PEG-catalase (500 U/ml), thioglycerol (5 mM) or CI-1033 (2 μM) for 30 min, and then exposed to NBD compounds (100 μM) or EGF (500 ng/ml) for 15 min. SOD1 monomers were present in all cells (shown only in cells pre-incubated with DMSO). (b) Detection of NBD compounds bound to purified human SOD1 with Coomassie staining. (c) Fluorescence detection of NBD compound/SOD1 complexes blotted onto NC membrane using a Typhoon 9410 scanner; the excitation wavelength was 488 nm and the emission wavelength was 526 nm.