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. 2016 Feb 17;6:20019. doi: 10.1038/srep20019

Figure 1. FXN mRNA is translated and processed to yield mature functional FXN in transfected cells.

Figure 1

(A) Schematic of the process used for mRNA generation. Linearized DNA template was used to generate the primary IVT product containing a cytomegalovirus (CMV) 5′ untranslated region (UTR) and a human growth hormone (hgh) 3′ UTR, and then subjected to 5′ capping and 3′ polyA tailing reactions to yield the final transcript. Bioanalyzer traces of IVT, capped product, and final polyA-tailed transcript are shown for (A) GFP and (B) FXN mRNA. (C) 293 T cells were transfected with the indicated amounts of FXN-6xHis or (D) FXN mRNA for 24 h before lysis and immunoblotting with the indicated antibodies. Anti-β-actin immunoblotting was used as a lane-loading control. p, i, and m indicate precursor, intermediate, and mature FXN protein immunopositive signals, respectively.