Table 1. Primer sequences and probes from Universal ProbeLibrary used in qPCR analysis.
Gene name | Primer sequences | Probe number |
---|---|---|
Runx2 | 5′-gtgcctaggcgcatttca-3′ | 29 |
5′-gctcttcttactgagagtggaagg-3′ | ||
Osterix | 5′-taacctgatggggtcatggt-3′ | 43 |
5′-gactgcagagcaggttcctc-3′ | ||
ColI A1 | 5′-cccctggaaagaatggagat-3′ | 60 |
5′-aatcctcgagcaccctgag-3′ | ||
Osteonectin | 5′-gtgcagaggaaaccgaagag-3′ | 77 |
5′-tgtttgcagtggtggttctg-3′ | ||
Vinculin | 5′-gatgaagctcgcaaatggtc-3′ | 28 |
5′-tctgcctcagctacaacacct-3′ | ||
α1 integrin | 5′-aattggctctagtcaccattgtt-3′ | 14 |
5′-caaatgaagctgctgactggt-3′ | ||
α2 integrin | 5′-tcgtgcacagttttgaagatg-3′ | 7 |
5′-tggaacacttcctgttgttacc-3′ | ||
α5 integrin | 5′-cccattgaatttgacagcaa-3′ | 55 |
5′-tgcaaggacttgtactccaca-3′ | ||
α11 integrin | 5′-gaggctgacgtcctcttcac-3′ | 66 |
5′-gttgggcttgacctcgtagt-3′ | ||
β1 integrin | 5′-cgatgccatcatgcaagt-3′ | 65 |
5′-acaccagcagccgtgtaac-3′ | ||
GAPDH | 5′-agccacatcgctcagacac-3′ | 60 |
5′-gcccaatacgaccaaatcc-3′ |
Abbreviation: Runx2, runt-related transcription factor 2; ColI A1, Type I collagen alpha1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.