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. 2016 Feb 17;6:21253. doi: 10.1038/srep21253

Table 1. Primer sequences and probes from Universal ProbeLibrary used in qPCR analysis.

Gene name Primer sequences Probe number
Runx2 5′-gtgcctaggcgcatttca-3′ 29
5′-gctcttcttactgagagtggaagg-3′
Osterix 5′-taacctgatggggtcatggt-3′ 43
5′-gactgcagagcaggttcctc-3′
ColI A1 5′-cccctggaaagaatggagat-3′ 60
5′-aatcctcgagcaccctgag-3′
Osteonectin 5′-gtgcagaggaaaccgaagag-3′ 77
5′-tgtttgcagtggtggttctg-3′
Vinculin 5′-gatgaagctcgcaaatggtc-3′ 28
5′-tctgcctcagctacaacacct-3′
α1 integrin 5′-aattggctctagtcaccattgtt-3′ 14
5′-caaatgaagctgctgactggt-3′
α2 integrin 5′-tcgtgcacagttttgaagatg-3′ 7
5′-tggaacacttcctgttgttacc-3′
α5 integrin 5′-cccattgaatttgacagcaa-3′ 55
5′-tgcaaggacttgtactccaca-3′
α11 integrin 5′-gaggctgacgtcctcttcac-3′ 66
5′-gttgggcttgacctcgtagt-3′
β1 integrin 5′-cgatgccatcatgcaagt-3′ 65
5′-acaccagcagccgtgtaac-3′
GAPDH 5′-agccacatcgctcagacac-3′ 60
5′-gcccaatacgaccaaatcc-3′

Abbreviation: Runx2, runt-related transcription factor 2; ColI A1, Type I collagen alpha1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.