Table 1. Gene-specific primers and amplicon characteristics of candidate reference genes and target genes used in qRT-PCR analysis.

Gene name	Length (bp)	Tm (°C)	Amplification efficiency (%)	R2	5′-sequence (forward primer)	3′-sequence (reverse primer)
18S	256	85.44	97.76	0.992	GCGGCTTAATTTGACTCAACACG	CCTGTTATTGCCTCAAACTTCC
28S	154	85.78	92.89	0.998	GCCGACCCTGATCTTCTGTGA	TACCCAAGTCAGACGAACGATT
ACTIN	149	82.27	93.34	0.996	CCAAAGGCCAACAGAGAGAAGAT	TGAGACACACCATCACCAGAAT
β-TUB	223	85.47	94.36	0.999	CCTTATTCCATTCCCCAGGCTTC	CATCTGCTCATCAACCTCCTTTGTGC
GAPDH	162	83.37	93.75	0.998	GTTTTCACTGACAAGGACAAGGCTGCTG	GGTGGTACAACTGGCATTGGAGAC
UBQ	127	81.39	93.18	0.999	GCTAAGATTCAAGACAAGGAGGGTATC	CCAGGTGGAGGGTTGATTCTTTCTG
PPDK	144	80.49	95.93	0.994	CTGTCCCAGGAGTCAAACAC	CACTGAACTAACTGCTTCCGA
SAT	168	88.35	97.12	0.994	GAGTATCTCCGAAGAGCCGAG	GCATCCTCACCATCCTTCTC