Table 2. Evaluation of intra-assay variation.

			Transduction titer (TU/ml)
	Vector genomes	Approximate MOI	Series 1	Series 2	Series 3	Average	SD	% CV
Assay 1	2.2 × 107	4.3	3.4 × 108	2.1 × 108	2.6 × 108	2.7 × 108	6.7 × 107	25
	7.3 × 106	1.0	1.9 × 108	1.4 × 108	1.2 × 108	1.5 × 108	3.2 × 107	21
	2.4 × 106	0.3	1.7 × 108	6.3 × 107	1.0 × 108	1.1 × 108	5.2 × 107	47
	1.2 × 106	0.1	1.6 × 108	6.4 × 107	9.6 × 107	1.1 × 108	4.8 × 107	45
	6.1 × 105	0.06	1.5 × 108	7.6 × 107	8.9 × 107	1.1 × 108	3.9 × 107	38
	3.1 × 105	0.04	1.5 × 108	ND	9.2 × 107	1.2 × 108	4.3 × 107	35
Assay 2	2.2 × 107	4.3	1.9 × 108	1.5 × 108	1.6 × 108	1.6 × 108	3.4 × 107	22
	7.3 × 106	1.0	1.4 × 108	1.4 × 108	1.3 × 108	1.3 × 108	1.7 × 107	13
	2.4 × 106	0.3	1.5 × 108	1.3 × 108	1.2 × 108	1.2 × 108	3.6 × 107	31
	1.2 × 106	0.1	1.1 × 108	1.2 × 108	1.0 × 108	1.0 × 108	2.0 × 107	20
	6.1 × 105	0.06	1.2 × 108	9.4 × 107	9.9 × 107	9.9 × 107	2.0 × 107	20
	3.1 × 105	0.04	1.2 × 108	9.0 × 107	1.1 × 108	1.1 × 108	1.7 × 107	15
Assay 3	2.2 × 107	4.3	1.6 × 108	1.6 × 108	1.6 × 108	1.6 × 108	1.2 × 106	0.7
	7.3 × 106	1.0	1.3 × 108	9.9 × 107	1.4 × 108	1.2 × 108	2.1 × 107	17
	2.4 × 106	0.3	1.0 × 108	1.1 × 108	1.3 × 108	1.1 × 108	1.3 × 107	11
	1.2 × 106	0.1	1.3 × 108	1.2 × 108	1.5 × 108	1.3 × 108	1.7 × 107	13
	6.1 × 105	0.06	1.2 × 108	1.1 × 108	9.0 × 107	1.1 × 108	1.6 × 107	15
	3.1 × 105	0.04	9.7 × 107	1.2 × 108	1.6 × 108	1.3 × 108	3.2 × 107	25

Multiple independent dilution series of a single vector sample were titered on one assay plate (series 1, series 2, and series 3). This assay was performed a total of three times on different days (assay 1, assay 2, and assay 3). Italicized numbers are outside the linear response range established in Figure 4c.

CV, coefficient of variation; MOI, multiplicity of infection; TU, transduction units.