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. 2015 Nov 17;44(3):1151–1160. doi: 10.1093/nar/gkv1183

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — HMGB1 depletion in human cells increases psoralen ICL-induced mutagenesis. (A and B) Western blot to assess siRNA-mediated depletion of HMGB1 in U2OS (TP53 proficient) and HeLa (TP53 deficient) cells, respectively. Lanes 1 and 2, untreated control; lanes 3 and 4, mock-siRNA treated; lanes 5 and 6, HMGB1-siRNA treated. (C and D) Spontaneous (P) and ICL-induced (P-ICL) mutation frequencies in HMGB1-depleted U2OS and HeLa cells, respectively. Cells were transfected with pSupFG1 plasmid (P) or with TFO-directed psoralen-crosslinked pSupFG1 (P-ICL), as indicated under the bars. The results are an average of four independent experiments; error bars represent ± SD. P-values derived (from one-way ANOVA (Holm-Sidak method).