Table 2. Laser microirradiation parameters and the associated biological observations over a range of input powers examined in our study with Mira-900 laser system.
| Input laser power (mW)* | In situ energy per pulse (nJ) | In situ peak irradiance (W/cm2) |
|---|---|---|
| 20 | 5.33 × 10−2 | 6.99 × 1010 |
| 25 | 6.67 × 10−2 | 8.74 × 1010 |
| 60 | 1.6 × 10−1 | 2.1 × 1011 |
| 85 | 2.27 × 10−1 | 2.97 × 1011 |
| 95 | 2.53 × 10−1 | 3.31 × 1011 |
| 100 | 2.67 × 10−1 | 3.49 × 1011 |
| 125 | 3.33 × 10−1 | 4.36 × 1011 |
| 155 | 4.13 × 10−1 | 5.41 × 1011 |
*This measurement is made on the laser beam prior to entry into the microscope. The subsequent columns in this table representing laser energy and irradiance that are calculated by determining the laser power entering the back aperture of the objective and then using the three objective method (see previous discussion in Materials and Methods section) determining the transmission through the objective.