Figure 2. Photomicrograph of native tissue (A and C), and decellularized matrix of human glans (B and D). Masson's Trichrome – A and B; Verhoff-Van Gieson – B and D revealed the preservation of collagen fibers (blue), architecture of muscle fibers (red), and elastic fibers (black), after the decellularization process. A - D (100X). Mf – Muscle fibers; Cf – collagen fibers; Ef – Elastic fibers; B - Blood vessels.