Figure 9. Representative profile of sperms in different staining techniques. (A) Acridine-orange staining for sperm DNA integrity: light green stained sperm is representing sperm cell with double strand DNA and the sperms with damaged DNA are presented with bright red stained nucleus; (B) Aniline-blue staining for chromatin condensation: the sperm with condensed chromatin is presented with light stained nucleus and the sperm with dark stained nucleus is representing de-condensed chromatin; (C); and (D) Eosin-nigrosin staining for sperm viability: live sperms are presented with colorless cytoplasm in figure (C) and the dead sperms are represented darkly stained cytoplasm in figure (D).