Fig. 3.

Production of the second generation GFP-mzNL4-3 virions. 72 hrs post-infection (p.i.) HEK293T cells infected with the first generation GFP-mzNL4-3 virions (50 μg of p24) produced syncytium and showed GFP emission (B1-B3), in contrast to control cells treated with the supernatant of pUC19-transfected cells (A1-A3). GFP-mzNL4-3 infection (0.5 μg of p24) resulted in the secretion of p24 antigen as efficient as infection with mzNL4-3 virions (C). Cells infected with 0.5 μg of either the first generation (G1) or the second generation (G2) GFP-mzNL4-3 virions were assayed for p24 production up to 20 days p.i. Contrary to G1, the amount of measured p24 for G2 infected cells was not higher than that for non-infected control cells (Neg) in different time points. Bars represent mean of p24 concentration ± SD in three infected wells (D).