Figure 7. HOTAIR Promotes CRPC Progression.

(A) HOTAIR regulation of androgen-dependent cell growth. Cell growth was assayed in LNCaP cells with control or HOTAIR overexpression and C4-2B cells with control or HOTAIR knockdown. Cells were grown in androgen-containing medium. Data shown are mean ± SEM of triplicate wells and are representative of at least two replicate experiments.

(B) HOTAIR regulation of androgen-independent growth of LNCaP and C4-2B cells grown in hormone-deprived condition is shown.

(C and D) HOTAIR overexpression increases LNCaP cell invasion. Cell invasion was monitored by Boyden chamber assay in LNCaP_Ctrl and LNCaP_HOTAIR cells. Representative images of invaded cells are shown (C), and the total number of invaded cells was quantified using colorimetry with absorbance at 560 nm (D). (E and F) HOTAIR depletion diminished C4-2B cell invasion as examined using Boyden chamber assay. Representative images of invaded cells are shown (E), and the total number of invaded cells was quantified using colorimetry with absorbance at 560 nm (F).

(G) The qRT-PCR showing PSA and HOTAIR expression in LNCaP cells following MDV3100 treatment. Gene expression was normalized to GAPDH. Error bars are mean ± SEM.

(H) Cell growth of C4-2B cells treated with MDV3100 alone or in combination with HOTAIR depletion is shown.