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. 2016 Feb 1;6:20094. doi: 10.1038/srep20094

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The formation of ¹O₂ was measured in non-heated and heated Chlamydomonas cells in the presence and the absence of catechol and caffeic acid using fluorescent probe SOSG. Heated cells were treated for 30 min in a water bath at 40 °C under dark. The images represent from left to right: Nomarski DIC, SOSG fluorescence, chlorophyll fluorescence and integral distribution of SOSG signal intensity (0–4096) in the 12-bit microphotographs.