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. 2015 Aug 17;35(17):2166–2177. doi: 10.1038/onc.2015.282

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Copyright © 2016 Macmillan Publishers Limited

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Pin1 is required for FOXM1 activity. (a) Expression of FOXM1 and CENPF is Pin1-dependent. Immunoblot analysis of the indicated proteins from lysates of wild type and Pin1⁻^/− MEFs synchronized in G0 by serum deprivation or induced to enter cell cycle progression by 10% FCS for the indicated intervals. N=Nocodazole-induced G2/M arrest. (b) Pin1^−/− MEFs show reduced FOXM1 expression under basal conditions. Immunocytochemical detection of Pin1 and FOXM1 in proliferating wild type and Pin1^−/− MEFs. The dashed lines indicate the contours of the nuclei. A merged image is shown to compare co-localization. (c) The induction of the FOXM1 target genes CENPF and Cylin-B1 by 4-OHT activatable FOXM1-ER is Pin1-dependent. U2OS cells stably expressing FOXM1-ER were transfected with control siRNA (siCtrl) or siRNA against Pin1 (siPin1). After 24 h they were exposed to 2 or 5μM 4-OHT and 24 h later processed for immunoblot analysis of the indicated proteins. Note that the ER-FOXM1 band is not significantly reduced by Pin1 knockdown, indicating stabilization due to the ER fragment.