Images of single- or dual-species biofilm formation in YPT-G medium by S. gordonii cells on saliva-coated cover slips with or without attached C. albicans SC5314 cells. Representative light microscopic fields of crystal violet-stained biofilms are shown. The middle panels show S. gordonii biofilms of the parent (top), ΔfruRBA mutant (middle) and fruRBA complemented (bottom) strains (DL1, UB2683, and UB2683/p49MfruRBA, respectively) grown from cells that bound during a 30-min incubation period to substrata with attached C. albicans cells. The flanking panels show corresponding monospecies biofilms of S. gordonii (left panels) grown from cells that bound saliva-coated substrata over 30-min incubation, and C. albicans biofilm (right panel) grown from cells that attached during 1-h incubation. Magnification Bar = 50 μm.