. Author manuscript; available in PMC: 2017 Aug 1.

Published in final edited form as: Mol Oral Microbiol. 2015 Sep 25;31(4):314–328. doi: 10.1111/omi.12125

Table 2.

Quantitative PCR validation of gene expression during S. gordonii and hyphal C. albicans interactions.

Locus	Gene Name	Relative Expression (2^−(ΔΔC_t))^a
Locus	Gene Name	Avg. Expression
Potentially Up-regulated by Microarray analysis
SGO_1111	fruR	6.5 ± 1.98
SGO_1112	fruB	9.05 ± 0.64
SGO_1113	fruA	3.75 ± 0.92
SGO_0701	hup	0.95 ± 0.21
SGO_0702	Hypothetical	1.95 ± 0.35
Potentially Down-regulated by Microarray analysis
SGO_1593	arcA	0.6 ± 0.28
SGO_1592	arcB	0.6 ± 0.28
SGO_1757	glmS	0.5 ± 0.14

Values were normalized to 150- or 100-bp amplicons of gyrA as appropriate. Expression values shown are those of DL1 cells in the hyphal interaction compared to baseline values (set at 1) of DL1 cells in pH-matched CSB-Glc. Relative expression values greater than 1 indicate up-regulation; values less than 1 indicate down-regulation. N=6.