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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Mol Oral Microbiol. 2015 Sep 25;31(4):314–328. doi: 10.1111/omi.12125

Table 2.

Quantitative PCR validation of gene expression during S. gordonii and hyphal C. albicans interactions.

Locus Gene Name Relative Expression (2−(ΔΔCt))a
Avg. Expression
Potentially Up-regulated by Microarray analysis
SGO_1111 fruR 6.5 ± 1.98
SGO_1112 fruB 9.05 ± 0.64
SGO_1113 fruA 3.75 ± 0.92
SGO_0701 hup 0.95 ± 0.21
SGO_0702 Hypothetical 1.95 ± 0.35
Potentially Down-regulated by Microarray analysis
SGO_1593 arcA 0.6 ± 0.28
SGO_1592 arcB 0.6 ± 0.28
SGO_1757 glmS 0.5 ± 0.14
a

Values were normalized to 150- or 100-bp amplicons of gyrA as appropriate. Expression values shown are those of DL1 cells in the hyphal interaction compared to baseline values (set at 1) of DL1 cells in pH-matched CSB-Glc. Relative expression values greater than 1 indicate up-regulation; values less than 1 indicate down-regulation. N=6.