Table 2.
Quantitative PCR validation of gene expression during S. gordonii and hyphal C. albicans interactions.
Locus | Gene Name | Relative Expression (2−(ΔΔCt))a
|
---|---|---|
Avg. Expression | ||
Potentially Up-regulated by Microarray analysis
| ||
SGO_1111 | fruR | 6.5 ± 1.98 |
SGO_1112 | fruB | 9.05 ± 0.64 |
SGO_1113 | fruA | 3.75 ± 0.92 |
SGO_0701 | hup | 0.95 ± 0.21 |
SGO_0702 | Hypothetical | 1.95 ± 0.35 |
Potentially Down-regulated by Microarray analysis | ||
SGO_1593 | arcA | 0.6 ± 0.28 |
SGO_1592 | arcB | 0.6 ± 0.28 |
SGO_1757 | glmS | 0.5 ± 0.14 |
Values were normalized to 150- or 100-bp amplicons of gyrA as appropriate. Expression values shown are those of DL1 cells in the hyphal interaction compared to baseline values (set at 1) of DL1 cells in pH-matched CSB-Glc. Relative expression values greater than 1 indicate up-regulation; values less than 1 indicate down-regulation. N=6.