| Immunoflourenscence microscopy |
ID |
Concentration |
Manufacturer |
| Primary |
Capillaries |
VECTB-1065 |
1:100 |
VWR, Bie & Berntsen, Herlev, Denmark |
|
Myofibre type I/IIA |
BF-35 |
1:50 |
Hybridoma Bank, Iowa City, IA, USA |
|
Myofibre Type I |
M8421 |
1:1000 |
Sigma-Aldrich, Brondby, Denmark |
|
Myofibre border/Laminin |
Z0097 |
1:500 |
Dako, Glostrup, Denmark |
| Secondary |
Streptavidin/FITC |
F0422 |
1:200 |
Dako, Glostrup, Denmark |
|
Alexa-488 donkey anti-mouse |
A21202 |
1:1000 |
Invitrogen, Life Technologies Denmark, Naerum, Denmark |
|
Alexa-555 donkey anti-mouse |
A31570 |
1:1000 |
Invitrogen, Life Technologies Denmark, Naerum, Denmark |
|
Alexa-350 goat anti-rabbit |
P10994 |
1:1000 |
Invitrogen, Life Technologies Denmark, Naerum, Denmark |
| Western blotting |
ID |
kDa |
Manufacturer |
| Primary |
Superoxide dismutase-2 |
AB10346 |
25 |
Calbiochem, Merck KGaA, Darmstadt, Germany |
|
Glucose Transporter-4 |
PA1-1065 |
42 |
Thermo Scientific, Hvidovre, Denmark |
|
Akt-2 |
3063 |
60 |
Cell Signalling Technology, Danvers, MA, US |
|
Mammalian target of rapamycin (mTOR) |
2972 |
289 |
Cell Signalling Technology, Danvers, MA, US |
|
Myostatin/GDF-8 |
sc-6885-R |
26 |
Santa Cruz Biotechnology, Dallas, TX, US |
|
Follistatin/H-114 |
sc-30194 |
35–70 |
Santa Cruz Biotechnology, Dallas, TX, US |
